PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Assays/Reagents Not for Customer Distribution
58
AEROSET
®
System Troubleshooting Guide
94816-107—November 2004
TRF
Transferrin + PEG/buffer (R1) + Antibody to transferrin (R2)
Turbidity due to transferrin:Antibody complex
PEG = Polyethylene glycol
Trig
Triglyceride + 3H
2
O Glycerol + 3 Fatty Acids
Glycerol + ATP Glycerol-3-phosphate + ADP
Glycerol-3-phosphate + O
2
DAP + H
2
O
2
H
2
O
2
+ 4-AAP + 4-CP Quinoneimine dye + 2H
2
O
ATP = Adenosine triphosphate
GK = Glycerol kinase
GPO = Glycerol phosphate oxidase
DAP = Dihydroxyacetone phosphate
4-AAP = 4-Aminoantipyrine
4-CP = Chlorophenol
The expected range
for Triglyceride stated
in the package insert
is < 200 mg/dL. The
low reference value
configured on the
Assay Configuration
screen is defined as 1
because the software
does not allow for a
“<” sign reference
value. Must not be on
the same line as the
Lipase assay.
Hyperlipemic samples
require a dilution to
be performed to
ensure accurate
results.
uAlb*
Microalbumin + Good’s buffer (R1) + TRIS buffer (R2) + Anti-human albumin
(goat) antibody (R2) Turbidity due to Microalbumin:Anti-human
albumin (goat) antibody complex (Insoluble)
The degree of
turbidity is
proportional to
albumin
concentration in the
specimen.
UPro
Native Protein + Benzethonium Chloride Denatured protein precipitate
(detected turbidimetrically)
This is a non-specific
method that does not
detect many proteins
in urine and CSF.
Urea
Urea + H
2
O 2NH
3
+ CO
2
NH
3
+ α-Ketoglutarate + NADH Glutamate + NAD
+
+ H
2
O
NADH = Nicotinamide adenine dinucleotide
GLD = Glutamate dehydrogenase
Assay
Name
Reaction Tips
* MULTIGENT
®
assay
Lipase
GK
GPO
Peroxidase
Urease
GLD
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