Abbott AEROSET - Page 59

PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution Assays/Reagents
AEROSET
®
System Troubleshooting Guide 59
94816-107—November 2004
Uric
Uric Acid + O
2
+ H
2
O Allantoin + CO
2
+ H
2
O
2
2H
2
O
2
+ 4-AAP + TBHB Quinoneimine + H
2
O
TBHB = 2,4,6 Tribromo-3-hydroxy benzoic acid
4-AAP = 4-Aminoantipyrine
Calibrate each reagent
cartridge. Run a
minimum of two
levels of control with
each new reagent
cartridge.
Ascorbate interferes
with this method.
Currently, Sigma
linearity standards
recover at
approximately 50%
for the highest level
on the AEROSET
System. The same is
seen with Roche
assays run on the
Roche/Hitachi.
Verichem linearity
was able to recover at
100%.
VPA*
Valproic Acid + Antibody to Valproic Acid/BIS-TRIS buffer (R1) +
Valproic Acid-coated Microparticles (R2)
Valproic Acid:Antibody complex + Valproic Acid-coated
Microparticle:Antibody complex
Valproic acid
concentration is
inversely proportional
to absorbance change.
(High absorbance
change = low valproic
acid concentration;
low absorbance
change = high
valproic acid
concentration.)
Assay
Name
Reaction Tips
* MULTIGENT
®
assay
Uricase
Peroxidase
Competition