Agilent Technologies 1290 Infinity II User Manual

1290 Infinity II High-Speed Pumps User Manual 95
5 Optimizing Performance
Using Solvent Calibration Tables
Recommendations for Setting up Methods Using
Salt-Containing Eluents
With the 1290 Infinity II Bio LC System Agilent offers a dedicated instrument for
running applications that are typical for the (Bio)pharma market, such as IEX (Ion
Exchange Chromatography)or HIC (Hydrophobic Interaction Chromatography),
and applications in Academia, Government and Applied Markets, using
concentrated salt solutions as eluents.
After you have imported the solvent tables, you may choose the solvent definition
most suiting your solvent according to the chart below:
Main salt component Total salt content (c, mol/L) Select solvent definition
NaCl
0.25 < c ≤ 0.75 Sodium Chloride 0.5M V.03
0.75 < c ≤ 1.25 Sodium Chloride 1.0M V.03
1.25 < c ≤ 1.75 Sodium Chloride 1.5M V.03
c > 1.75 Sodium Chloride 2.0M V.03
(NH
4
)
2
SO
4
0.25 < c ≤ 0.75 Ammonium Sulphate 0.5M V.03
0.75 < c ≤ 1.25 Ammonium Sulphate 1.0M V.03
1.25 < c ≤ 1.75 Ammonium Sulphate 1.5M V.03
1.75 < c ≤ 2.5 Ammonium Sulphate 2.0M V.03
c >2.5 Ammonium Sulphate 3.0M V.03

Agilent Technologies 1290 Infinity II Specifications

General

Brand	Agilent Technologies
Model	1290 Infinity II
Category	Laboratory Equipment
Language	English