1290 Infinity II High-Speed Pumps User Manual 61
3Using the Pump
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260
Infinity II Flexible Pumps
Solvent Handling
Handling of Normal Phase Solvents
Observe the following recommendations when using normal phase solvents:
• Always use fresh, filtered solvents. Exchange solvents every second day.
• Prevent reactions caused by heat, light and oxygen. Use brown, firmly closed
bottles.
• Whenever possible, use stabilizers, e.g. butylated hydroxytoluene (BHT) for
ethyl ether.
• Use isopropanol (IPA) to flush out the previous solvent when converting a
system from normal phase to reverse phase, or vice versa.
• Store all unused channels in IPA.
• Don’t turn off the pump while it is filled with solvents with low boiling points.
Generate a small flow throughout the used channels when the pump is not in
use.
Handling of Buffers
The following recommendations should be observed when using buffer
solutions:
• Buffers and aqueous solutions are possible sources of algae contamination,
for avoiding related problems, please read “Algae Growth in HPLC
Systems” on page 64.
• For buffer concentrations of 0.1 M or higher using the seal wash function
periodically with a runtime of 0.3 min every 3 min is strongly recommended.
• Filter buffer solutions to avoid increased wear or blockages that are caused
by undissolved crystals. Always use solvent inlet filters.
• Avoid conditions where mixing of buffers and organic solvents may cause
precipitation, as this impairs the reproducibility of chromatographic
experiments and may also reduce the system life time. For example in
reversed phase chromatography, avoid buffers (especially phosphate buffers)
with a concentration higher than 20 mmol/L. For phosphate buffers, avoid
compositions containing more than 65 % acetonitrile or other organic
solvents.
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