1290 Infinity II High-Speed Pumps User Manual 39
3Using the Pump
Best Practices
Power up / Shut-down the pump
Prepare the Pump
• Use fresh or different mobile phase (as required).
• Purge each channel with 2.5–3mL/min for 5 min. Open the manual purge
valve or use the purge command, depending on the pump type.
Shut Down the System
• Flush the column with the appropriate solvents and store it according to
column manual instructions (be sure that the flushing solvent is compatible
with the solvent present in the system to avoid precipitation).
• Install a union or a restriction capillary and flush the system extensively with
water, especially after using buffers. For details, see “Flushing Procedure” on
page 48.
• Flush and store the system in 50 % methanol or 50 % isopropanol in water,
without additives.
• Remove all samples from the autosampler and store them according to good
laboratory practices.
• Power off all modules.
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