Agilent Technologies 7200 - Calibrating the GC Column

58 Operation Manual
3 Operating in Electron Impact (EI) Mode
To Calibrate the Column
3 Check the heater On and enter the setpoint in the Value °C
column. The typical setpoint is 280 °C. The limits are 0 °C to
400 °C. A setpoint below ambient temperature turns off the
interface heater.
4 Click Apply to download setpoints or click OK to download
setpoints and close the window.
5 To make the new settings part of the method, select Save from
the Method menu.
To Calibrate the Column
Capillary columns must be calibrated prior to use with the MS.
Procedure
1 Set Data Acquisition for splitless manual injection and set up
a real time plot to monitor m/z 28.
2 Press [Prep Run] on the GC keypad.
3 Inject 1 µL of air into the GC inlet and press [Start Run]
4 Wait until a peak elutes at m/z 28. Note the retention time.
5 In the Instrument Control panel, select .
6 Select the Configuration tab.
7 Select the Column tab and click on the Inventory button and
verify that the column you are using is in inventory. Select
the column to be calibrated and click Install Selected Column.
8 Highlight the column in the inventory list and select the
Calibrate button.
9 Select the Calc Length button.
10 In the Calculate Column Length dialog box, enter the recorded
retention time in the Holdup Time field. Verify that the other
CAUTION
When setting the GC/MS interface temperature, never exceed the
maximum for your column.