5 Micro GC Channels
Tuning the backflush time (except on a HayeSep A channel)
Agilent 990 Micro GC User Manual 77
Tuning the backflush time (except on a HayeSep A
channel)
Tuning the backflush time is necessary for each new channel. This chapter describes how to tune
the backflush time on all channels except HayeSep A.
Tuning procedure for the backflush time
1 Set the backflush time to 0 seconds, and analyze the checkout sample or a proper sample for
the specific channel. The goal is to identify the components in the calibration standard.
2 Change the backflush time to 10 seconds and perform a run. The following can be observed:
• When the backflush time is set too early, the peaks of interest are partially or totally
backflushed.
• If the backflush time is set too late, the unwanted components are not backflushed and
show up in the chromatogram.
3 Perform runs with different backflush times until there is no significant difference in the peak
of interest. To fine tune the backflush time, set smaller steps (for example 0.10 seconds) until
you find the optimal backflush time.
Figure 29 shows a simple example of tuning the backflush time for the CP-Molsieve 5A channel.
Figure 29. Effect of the backflush time on the peak of interest
Tuning backflush for CP-Molsieve channel
Area_peak of interest
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