4Sample Gas Handling
Manual Injection
56 Agilent 990 Micro GC User Manual
Manual Injection
Manual injection is possible with the sample inlet that can accommodate 1/16-inch tubing.
Manual injection guidelines
• Use sample pump mode, and set sampling time of 10 to 20 seconds in the method. This
clearly marks when injector loop is flushed (sound of the pump). Then gently push the syringe
during that period.
• Flush the sample path 6 to 10 times. Bulkhead union, additional tubing, pressure relieve valve,
and ball valve adds dead volume to the system, estimated at 500 to 1,000 µL.
• Total sample volume is dependent on the internal volume of the Micro GC and the number of
times flushed and the sampling time in the method.
Injection procedure
1 Do not use continuous flow mode.
2 Minimum sample volume should be > 10 mL.
3 Insert or connect syringe and start the run.
4 Gently inject when pump starts to aspirate.
When performing manual injection with a luer lock valve, use a10 mL gas tight syringe
(p/n 5190-1543: syringe 10 mL, PTPE, luer lock valve).
There may be unique syringe requirements when performing Septum nut injection.
The manual syringe injection would lead to increase the repeatability (RSD%) compared to
automated pump or continuous flow mode.
To Next Page
Loading...
Need help?
General
