Applied Biosystems 2720 User Manual

4-12 Methods and Users
Inserting a Cycle
A cycle is a PCR portion of a method usually consisting of three time/temperature
parameters and delineated by dashed lines. The instrument repeats these three
temperatures and times for the number specified in the Cycles field (default is 25).
3 Press F3 (Delete).
The Insert-Delete-More screen redisplays minus the hold you deleted.
Note The Modify function (F1) also displays if the next highlighted parameter was
in the PCR segment.
4 Press F4 (More) to return to the Create/Edit screen.
To delete a hold: (continued)
Step Action
3 Tmp
25 Cycles 2 Holds
94.0
0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
MoreDeleteInsert
Modify
To insert a cycle:
Step Action
1 From the Create/Edit screen, use the arrow keys to highlight a time or temperature
parameter to the left of which you want to insert a cycle.
2 Press F4 (More).
The Insert-Delete-More screen appears.
Note The Modify function (F1)also displays if the highlighted parameter was in
the PCR segment.
3 Press F2 (Insert).
The Insert screen appears.
Note The Pause function will not display on the Insert screen if the highlighted
segment already has a pre-programmed pause or if highlighter is not on a PCR
segment parameter.
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0
94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
MoreDeleteInsert
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0
94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
Hold CancelPauseCycle

Applied Biosystems 2720 Specifications

General

Brand	Applied Biosystems
Model	2720
Category	Laboratory Equipment
Language	English