Applied Biosystems 2720 User Manual

4-14 Methods and Users
Inserting a
Programmed Pause
A programmed pause is a point in the PCR portion of a method when cycling stops for
a specified length of time. You define the frequency and length of the pause and the
cycle at which it begins.
To insert a programmed pause:
Step Action
1 From the Create/Edit screen, use the arrow keys to highlight a time or temperature
parameter of the PCR segment during which you want to insert a programmed
pause.
2 Press F4 (More).
The Modify screen appears.
3 Press F2 (Insert).
The Insert screen appears.
4 Press F3 (Pause).
The Define Programmed Pause screen appears.
The fields that can be changed are highlighted in the illustration here.
5 Type in the values you want to change. When you move the highlighter to No, the
functions F2 (Yes) and F3 (No) become active. Press the appropriate key.
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0 94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
Modify MoreDeleteInsert
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0 94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
Hold CancelPauseCycle
Define Programmed Pause
Start first pause at cycle 1 of 25.
Pause every 25 cycles for 00:30.
F1 F3 F4 F5F2
Accept Cancel

Applied Biosystems 2720 Specifications

General

Brand	Applied Biosystems
Model	2720
Category	Laboratory Equipment
Language	English