Applied Biosystems 2720 User Manual

4-16 Methods and Users
Deleting a
Programmed Pause
Auto-Incrementing/
Decrementing
Temperature
Control Parameters
Using the AutoX function, you can automatically increase or decrease the value for
any PCR segment parameter a fixed amount every cycle.
Note This feature is particularly useful towards the end of the amplification process since the
amount of PCR product, available to be extended, increases with the number of cycles while the
amount of available enzyme remains constant.
To delete a programmed pause:
Step Action
1 From the Create/Edit screen, use the arrow keys to highlight the word Pause.
2 Press F4 (More).
The Insert-Delete-More-Edit screen appears.
3 Press F3 (Delete).
The Modify screen appears without the pause.
4 Press F4 (More) to return to the Create/Edit screen.
1 Hld 4 Tmp
25 Cycles
2 Holds
94.0
94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
MoreDeleteInsert
Edit
0:30
Pause
To automatically increase or decrease temperature control parameters:
Step Action
1 Use the arrow keys from the Create/Edit screen to select a time or temperature
parameter in the PCR segment.
2 Press F4 (More).
The Modify screen appears.
3 Press F1 (Modify).
The Select Modification screen appears.
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0 94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5F2
Modify MoreDeleteInsert
Select Modification
F1 F3 F4 F5F2
AutoX Cancel
- Increments or decrements time
or temperature at the
completion of each cycle
AutoX

Applied Biosystems 2720 Specifications

General

Brand	Applied Biosystems
Model	2720
Category	Laboratory Equipment
Language	English