Applied Biosystems 7500 - Page 123

Chapter 7 Verifying the Instrument Performance
Analyzing the TaqMan
®
RNase P Plate Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 97
Notes
7
12. Calculate the verification value for the
5000-copy population:
a. In the Report tab, scroll to a sample in the
5K population.
b. Apply the values in the Mean Qty and
StdDev Qty columns to the following
equation:
5K value = Mean Qty + 3(StdDev Qty)
13. Calculate the verification value for the
10000-copy population:
a. In the Report tab, scroll to a sample in the
10K population.
b. Apply the values in the Mean Qty and
StdDev Qty columns to the following
equation:
10K value = Mean Qty – 3(StdDev Qty)
14. Compare the verification values for the 10000-
and 5000-copy populations:
• If the 10K value (step 13) is greater than the
5K value (step 12), the 7300/7500/7500
Fast system has passed the installation
verification.
• If the 10K value is not greater than the 5K,
the 7300/7500/7500 Fast system has failed
the installation verification. Troubleshoot
the RNase P verification run. See
“Troubleshooting” on page 99.
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