Error Detection Limits
Initial Rate High and Substrate Depletion
UniCel DxC Systems Instructions For Use A13914 User Defined Reagents
October 2005 Page 9-17
9
Initial Rate High and Substrate Depletion
The initial rate parameter specifies maximum rate of absorbance change measured
within the first cycle after the last component (sample or reagent) is added. When this
value is exceeded, results will be suppressed and flagged Initial Rate High (INT RATE
HI).
The delta absorbance specifies the maximum acceptable difference between the
Reaction HIGH ABS, measured just after sample or starter reagent addition, and the
final absorbance measured at the end of the reaction read window. When this value is
exceeded, results will be suppressed and flagged Substrate Depletion (SUB DEPL).
Note: For downgoing reactions, the initial rate should be a negative number.
Multipoint Spans
Multipoint span limits specify the allowable difference in absorbance or rate between
respective multipoint calibrator levels:
• 1 and 2.
• 2 and 3.
• 3 and 4.
• 4 and 5.
• 5 and 6.
• 6 and 1.
Values will only be shown for the number of calibrators specified under "# of
Calibrators". A change in calibrator number will reset the spans to zero (0.000).
The sign (positive or negative) of multipoint spans are dependent upon the direction of
change between two successive calibrators. For example, if the response for Cal 2
data < Cal 1 data, the multipoint span must be negative.
Table 9.29 Allowable Entries for Initial Rate and Delta Absorbance
Initial Rate -99.999 to 99.999
Delta Abs 0.000 to 2.200
Table 9.30 Allowable Entries for Multipoint Span Limits
-1.500 to 2.200
NOTICE
The system may accept incorrect calibration data if:
• multipoint span values are not entered during UDR setup, and
• the samples are not loaded correctly during the run.
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