Cartridge Chemistry: Principles of Measurement
Spectrophotometric Methods
System Description UniCel DxC Systems Instructions For Use A13914
Page 2-50 October 2005
Cartridge Chemistry: Principles of Measurement
Spectrophotometric Methods
Spectrophotometric methods rely on the principle that a sample, such as a patient
sample, a control, or a calibrator, when mixed with one or more appropriate chemical
reagents, produces a substance that has the ability to absorb light at specific
wavelengths. This substance is referred to as a chromophore.
Beer's Law
According to Beer's Law, the amount of light absorbed by the chromophore is
proportional to the concentration of the constituent being measured. The system can
measure this as an endpoint or a rate of formation.
A = abc
where
A = absorbance of the chromophore
a = absorptivity of the absorbing substance at the specific measuring
wavelength(s)
b = cuvette light pathlength (cm)
c = constituent concentration (M)
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