Skyla HB1 - Sample Preparation

3-2

The center of the reagent disc contains a
tightly
sealed sample diluent container
. Prior to use, the
aluminum foil strip of the dilu
ent container that
covers the sample anti-
spilling pad must be removed
to ensure the normal operation of the
clinical
chemistry analyzer.

During analysis, the clinical chemistry a
nalyzer first
performs a centrifugation step, where
the plasma and
blood cells are separated in the reagent disc i
f the
sample is a whole-blood sample. This
step has no
effect on plasma or serum samples. Next, accurately
quantified sample and diluent
are delivered to the
mixing chamber for dilution. The fully
diluted
sample is then distributed to all reaction cuvettes
in
the disc by centrifugal force, where
the chemical
reactions with assay reagents occur. Clinical
chemistry markers are quantified by photometric
measurement of the absorbance changes arising from
these chemical reactions.
The reagent disc features internal quality control
functions in
order to ensure adequate sample and
diluent are properly distributed
to every reaction
cuvette in the disc. In addition,
possible sample
interferences that might
affect the test results are
automatically monitored
. If the degree of interference
exceeds the specification limit
of relevant markers
under analysis,
the system will display a warning
message.
Sample Conditions:
 Use serum or lithium-
heparinized whole-blood or
plasma samples.
3.2 Sample Preparation
The compatible sample types for the skyla
TM
Clinical
Chemistry Analyzer include: serum, lithium-
heparinized
whole blood or plasma.