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For fluorescence, he standard test time is 0.2 seconds.
Note: in the case the au-exposure does not give you entire satisfaction for your specific application,
we recommend increasing the test time.
Auto mode
The Auto mode options allow you to select between a standard auto exposure time, an extended
auto exposure time or a reduced auto-exposure time.
Standard exposure is optimized for all bands.
Over exposure (faint band) results in a longer exposure where faint bands are more visible, but more
prominent bands may be over exposed.
Under exposure (bright band) results in a shorter exposure where bright bands are more visible, but
more prominent bands may be under exposed.
Manual exposure set-up
Manual exposure set-up
This option defines the default imaging exposure time.
For the fluorescence mode, the FireReader 1D has two exposure time scales:
- One for short times: 80 milli-second to 4 seconds
- One for long times: 4 sec to 1 minute
Note: With the short integration time scale, the integration time increases or decreases by 40milli
seconds.
Note
: With long integration time, a delay could be necessary before an image is displayed on the
monitor (up to twice the selected Exposure time).
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