Pannoramic 250 1.15 User’s Guide 3 Operating Slides and Magazines
3 Operating Slides and Magazines
3.1 Preparing Slides and Magazines
This section demonstrates how to prepare slides and magazines.
You can use all slides and cover slips that meet the following specifications:
Slides Cover slips
Length 74.9 to 76.2 mm max. 50 mm
Width 24.6 to 26.1 mm max. 24 mm (recommended: 22 mm)
Thickness 0.9 to 1.2 mm No. 1 and No. 1.5 (0.13 to 0.16 mm and 0.16 to 0.19 mm)
Corners 45° beveled or 90° cut corners
Edges Grounded or cut
Note:
FISH fluorescent signals become more easily detectable for the
scanner, therefore resulting in a brighter and better image, by treating
the slide with a formula that prevents rapid photobleaching of
fluorescent reagents (e.g. VECTASHIELD® Fluorescent Mounting
Medium by Vector Laboratories and ProLong® Gold and SlowFade®
Gold antifade reagents by Invitrogen) during the FISH protocol.
For further information, see the homepages of the manufacturers:
http://www.vectorlabs.com/ and http://www.invitrogen.com.
Note:
The distance between slide edges and the edges of the mounted
coverslip should be 1-2 mm, and they should be parallel with each
other. Barcode stickers thicker than 0.4 mm must not be used.
March 13, 2012 – Rev. 1 3DHISTECH Ltd.
27(135)
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