Methods Performance C-5
6The results are stored in the analyzer. Print the
results, transmit to a computer, or press OPEN to
bypass this step. To transmit, see Section 11, “Con-
necting to an External Computer” for instructions.
7The drawer automatically opens. Remove the rotor
from the drawer and follow standard hazardous
waste disposal procedures when disposing the rotor.
The rotor may be placed back into the pouch before
disposal. Press CLOSE if another reagent rotor is
not to be run immediately.
C.10 Timing Considerations
• Analyze whole blood samples collected by venipunc-
ture within 60 minutes of collection.
•Run the reagent rotor within 10 minutes of applying
sample or control to the rotor.
•Dispense samples collected in micropipettes into the
rotor immediately after collection.
C.11 Quality Control
Although elaborate quality controls are integrated into the
VetScan VS2 Chemistr y Analyzer and reagent profile
rotors, the analyzer’s performance can also be verified by
running external controls. This can be accomplished by
testing an aliquoted sample with known values, or by
using a serum-based, commercially available control.
See Section 7.3, “Running Controls” to run controls.
C.12 Calibration
The VetScan VS2 Chemistry Analyzer is calibrated by the
manufacturer before shipment. The bar code printed on
the bar code ring provides the analyzer with rotor-specific
calibration data. See Section 7 for details.
C.13 Results
The VetScan VS2 Chemistry Analyzer automatically cal-
culates and prints the analyte concentrations in the sam-
ple. Details of the endpoint and rate reaction calculations
are found in Section 9.
The VetScan analyzer calculates the globulin concentra-
tion using the total protein and albumin concentrations
determined by the chemical analyses. If the total protein
or albumin results are out of range or suppressed, the
globulin concentration cannot be reliably calculated and
is not reported.
C.14 Messages Printed in Results
•Results outside the reference range are indicated in
the results by an asterisk ( * ) next to the analyte con-
centration.
•Results outside the dynamic range are indicated in the
results by a greater-than symbol ( > ) next to the high-
est value of the dynamic range, or a less-than symbol
( < ) next to the lowest value of the dynamic range. (For
example, concentrations outside an analyte dynamic
range are printed as >700 or <10, respectively.)
•“HEM”, “LIP”, or “ICT” is printed in place of the analyte
concentration if hemolysis (HEM), lipemia (LIP), or ict-
erus (ICT) adversely affects the results for that analyte.
LIP is also printed if both lipemia and icterus affect a
result. HEM is also printed if hemolysis and icterus,
hemolysis and lipemia, or hemolysis, lipemia, and ict-
erus affect a particular analyte. Examine the sample
indices to determine if more than one interferent is
affecting a particular result.
•The sample indices are printed in the results. The indi-
ces indicate the degree of hemolysis, icterus, and
lipemia in the sample, measured on a scale of 0
(clear), 1+ (slight), 2+ (moderate), and 3+ (gross).
C.15 Limitations of Procedure
•Lithium heparin is the only anticoagulant recom-
mended for use with the VetScan VS2 Chemistry Ana-
lyzer.
•If a result for a particular test exceeds the assay range,
the sample may be able to be diluted (see Section 8). If
the sample again appears out of range, have it ana-
lyzed by another approved test method or sent to a
referral laboratory. Do not dilute the sample and run it
a third time.
•If sample or reagent rotor errors are indicated on the
analyzer display or printed in the results, consult
Section 8 for an explanation of the error message.
• Samples with hematocrits in excess of 62–64% packed
red cell volume (a volume fraction of 0.62–0.64) or that
are hemolytic, lipemic, or icteric may give inaccurate
results. Samples with high hematocrits may be
reported in the results as being hemolyzed. If hemoly-
sis, lipemia, or icterus adversely affect results, a mes-
sage will be printed in the results in place of the
analyte concentration (see Section 8).
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