Applied Biosystems 7300 User Manual

Chapter 1 Before Starting the Installation
Selecting the Installation Method
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 3
Notes
1
• Performing pure dye calibrations
• Verifying instrument performance
Using the Wizard
to Recalibrate the
System
You can also use the Real-Time System Setup wizard after the system is up and running,
to perform the routine recalibrations and verification runs that ensure optimum
performance:
Access the wizard by selecting Program Files 7300/7500/7500 Fast
System Real-Time System Setup Wizard.
Recalibration/
Verification
Purpose When to Perform
ROI Defines the well positions on the sample block. • Every 6 months
• After replacing the halogen lamp
After an ROI recalibration, you must also
perform a background, optical (7500/7500
Fast systems only), pure dye calibration, and
instrument verification.
Background Measures the level of background fluorescence in the
instrument. During a run, the software removes the
background fluorescence from the run data.
• Once a month
• After replacing the halogen lamp
After a background recalibration, you must
perform an optical (7500/7500 Fast systems
only).
Optical
(7500/7500 Fast
systems only)
Compensates for the physical effects of the additional
filter present in 7500/7500 Fast instruments.
• Once a month
• After performing a background calibration
• After replacing the halogen lamp
Pure Dye Characterizes each dye. During a run, the software
uses the pure dye calibration spectra to distinguish the
individual contribution of each dye in the collective
fluorescence gathered by the instrument.
Every 6 months
Before performing, you must perform a
background calibration (7300 systems) or
optical calibration (7500/7500 Fast systems);
RNase P Verifies that the instrument can distinguish between
5,000 and 10,000 genome equivalents of the RNase P
gene with a 99.7% confidence level.
After moving the instrument to another
location or as needed to verify the function of
the instrument

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English