Applied Biosystems 7300 - When to Verify Instrument Performance; Purpose of RNase P Runs

Chapter 7 Verifying the Instrument Performance
Overview
88 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Overview
Note: You can also use the Real-Time System Setup wizard to perform this procedure.
To access the wizard, select Program Files 7300/7500/7500 Fast
System Real-Time System Setup Wizard.
Time Required • 7300/7500 systems – ~ 2 hours
• 7500 Fast systems – ~ 40 minutes
Materials
Required
When to Verify
Instrument
Performance
Applied Biosystems recommends running a TaqMan
®
RNase P Instrument Verification
plate:
• When installing the system (you must perform all calibrations in this order: ROI
backgroundoptical (7500/7500 Fast systems only)pure dyeinstrument
verification).
• After moving the instrument to another location.
• As needed to verify the function of the 7300/7500/7500 Fast instrument.
Purpose of
RNase P Runs
The TaqMan RNase P Instrument Verification Plate run verifies the performance of an
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System. The RNase P plate is
pre-loaded with the reagents necessary for the detection and quantification of genomic
copies of the human RNase P gene (a single-copy gene encoding the RNase moiety of
the RNase P enzyme). Each well contains:
• Reaction mix:
– 7300/7500 systems: 1✕ TaqMan
®
Universal PCR Master Mix
– 7500 Fast systems: 1✕ TaqMan
®
Fast Universal PCR Master Mix
• RNase P primers
•FAM
™
dye-labeled probe)
• Known concentration of human genomic DNA template
Powder-free
Gloves
Safety
Goggles
Ta qM an
®
RNase P
Instrument Verification
Plate
Taq Ma n
®
RNase P Instrument
Verification Plate Kit
GR2170
Centrifuge with plate
adapter