Chapter 4 Performing the Regions of Interest (ROI) Calibration
Performing the ROI Calibration
44 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Performing the ROI Calibration
1. In the ROI Inspector dialog box:
a. In the Exposure Time field, enter 2048.
b. Select Filter A.
2. Click to generate an ROI image.
3. Determine if your ROI image is acceptable (the
figures to the right show an unsaturated image
and an oversaturated image). Wells in an
acceptable image:
• Must be as bright as possible without
oversaturating. (When you generate the ROI
calibration on page 45, a warning is
displayed if wells are oversaturated).
• Can contain some, but do not have to
contain any, red pixels, which represent
saturation.
4. If your ROI image is acceptable, skip to step 5.
If your ROI image is oversaturated, decrease the
Exposure Time by half, then click
. Repeat until you obtain an
acceptable ROI image.
If you cannot obtain an acceptable image, see
“Troubleshooting” on page 48.
b
a
Unsaturated ROI image
Over-saturated ROI image
Unsaturated
Normal
Saturation
Over
Saturated
Good Acceptable Bad
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