Applied Biosystems 7300 User Manual

Chapter 6 Performing the Pure Dye Calibration
Overview
68 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Overview
Note: You can also use the Real-Time System Setup wizard to perform the procedure in
this chapter. To access the wizard, select Program Files 7300/7500/7500
Fast System Real-Time System Setup Wizard.
Time Required • 7300 systems: 45 minutes
• 7500/7500 Fast systems: 1hour
Materials
Required
When to Perform
Pure Dye
Calibrations
Perform a pure dye calibration:
• When installing the system (you must perform all calibrations in this order: ROI
backgroundoptical (7500/7500 Fast systems only)pure dyeinstrument
verification).
• Every 6 months, depending on instrument use.
IMPORTANT! You must perform a background run before every pure dye calibration.
Because the age and use of instrument components can affect pure spectra readings,
Applied Biosystems recommends performing a pure dye calibration at least every six
months.
Safety
Glasses
Powder-free
Gloves
Pure Dye Plates
(FAM
™
, JOE
™
, NED
™
,
ROX
™
, SYBR
®
Green,
TAMR A,
™
and VIC
®
dyes)
Pure Dye Plates
(CY3, CY5, and
TEXAS RED
®
dyes)
7300, 7500/7500
Fast system
7500/7500 Fast
system only
Applied Biosystems
Real-Time PCR System
Spectral Calibration Kit
GR2170
Centrifuge with plate
adapter

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English