Applied Biosystems 7300 - Performing the Background Calibration

Chapter 5 Performing the Background Calibration and Optical Calibration
Performing the Background Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 55
Notes
5
Performing the Background Calibration
1. Load the plate in the instrument as described in
“Loading the Plate” on page 40.
Note: If you cannot open the tray, the sample
block may be in its raised position, locking the
tray position. To lower the block, select
InstrumentCalibrate, then exit the ROI
Inspector.
2. In the SDS software:
a. Select the Instrument tab.
b. Click .
The instrument begins the background
calibration run.
Note: Before starting the run, the instrument
may pause (up to 10 minutes) to allow the heated
cover to reach the correct temperature.
Continue with “Analyzing the Background
Calibration Data” on page 56.
Notched
corner
Well A1
12
GR2475
GR2475
7500
keyed corner
A1
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B
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1
7300/7500 System
Standard plate
7500 Fast System
Fast plate
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b