Applied Biosystems 7300 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Analyzing the Background Calibration Data
56 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Analyzing the Background Calibration Data
1. When the run is complete, click .
2. Click (or select AnalysisExtract
Background).
The software extracts the background signal,
then displays one of the following messages:
Click
Select
If the software displays: Do this:
Background Extraction Complete – The analysis is
successful.
Click , then go to step 4.
Image exposure is too low… – The software stopped
the extraction because several raw spectra are at or
below the detectable threshold for the calibration.
a. Click .
b. Verify that the instrument contains the background
plate.
c. Test the lamp. See “Monitoring Lamp Status” on
page 117.
d. Run the background plate again.
e. If the SDS software continues to display the Image
exposure is too low… dialog box, click ,
then go to step 4.
Image exposure is too high… –The run is
unsuccessful. The software stopped the extraction
because one or more raw spectra exceed the maximum
limit for the 7300/7500/7500 Fast system.
Click , then troubleshoot the failed run. See
“Troubleshooting” on page 64.
Click

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English