Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 141
Index
Numerics
7500/7500 Fast system optical calibration
about 59
creating the plate document 61
materials required 59
performing 59, 62
preparing the plate 59
time required 59
when to perform 59
A
analyzing
background data 56
optical calibration data (7500/7500 Fast systems
only) 63
pure dye calibration data 77
RNase P plate data 94
ROI data 44
antivirus software 10
Applied Biosystems
contacting xiii
customer feedback on documentation xii
Information Development department xii
Technical Support xiii
archiving SDS files 107
assumptions for using this guide xi
B
background calibration
analyzing the data 56
creating the plate document 54
materials required 50
performing 55
purpose 50
time required 50
when to perform 50
background component, about 51
background plate
number of reuses 52
preparing 52
running 55
backing up SDS files 107
backup storage devices 10
biohazardous waste, handling xxiii
biological hazard guidelines xxiv
C
calibration
background 50
optical (7500/7500 Fast systems only) 59
pure dye 67
ROI 37
CAUTION, description xvi
checking, shipped materials 14
chemical safety xxi, xxii
chemical waste safety xxii
chemistry kits, shipped with the instrument 17
cleaning the sample block 108
computer
checking 14
cleaning up the disk 114
connecting to a network 10
defragmenting the hard drive 114
setting up 2, 20
computer, wireless network, do not use 10
contamination
decontaminating the sample block 108
locating on the sample block 64
conventions
IMPORTANTS! xii
in this guide xi
Notes xi
safety xvi
user attention words xi
creating
background plate document 54
pure dye plate document 72
RNase P plate document 92
custom pure dyes, adding to the pure dye set 131
customer feedback, on Applied Biosystems
documents xii
CY3 pure dye 69, 83, 85
CY5 pure dye 69, 83, 85
D
damage, checking and reporting 20
DANGER, description xvi
data management 107
decontaminating the sample block 108