Chapter 5 Performing the Background Calibration and Optical Calibration
Overview
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 51
Notes
5
The software then uses the calibration file during subsequent runs to remove the
background fluorescence from the run data.
Background
Fluorescence
Fluorescence data collected by the Applied Biosystems 7300/7500/7500 Fast Real-Time
PCR System includes a fluorescent signal inherent to the system, commonly referred to
as background fluorescence. Background fluorescence is a composite signal found in all
spectral data. This signal consists of fluorescence from several sources, including:
• Background electronic signal
• Contaminants in the sample block
• The plastic consumable (plates and caps)
Guidelines for
Calibration
For a new instrument:
• Always start with a new calibration kit.
• Make sure the centrifuge you use is clean. Before centrifuging, wipe down the
bucket using a tissue.
• Handle the calibration plates with care to prevent contamination. Do not place
plates on a lab bench, which may contaminate the plate. Always put calibration
plates back into their original bags.
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