Applied Biosystems 7300 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Overview
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 51
Notes
5
The software then uses the calibration file during subsequent runs to remove the
background fluorescence from the run data.
Background
Fluorescence
Fluorescence data collected by the Applied Biosystems 7300/7500/7500 Fast Real-Time
PCR System includes a fluorescent signal inherent to the system, commonly referred to
as background fluorescence. Background fluorescence is a composite signal found in all
spectral data. This signal consists of fluorescence from several sources, including:
• Background electronic signal
• Contaminants in the sample block
• The plastic consumable (plates and caps)
Guidelines for
Calibration
For a new instrument:
• Always start with a new calibration kit.
• Make sure the centrifuge you use is clean. Before centrifuging, wipe down the
bucket using a tissue.
• Handle the calibration plates with care to prevent contamination. Do not place
plates on a lab bench, which may contaminate the plate. Always put calibration
plates back into their original bags.

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English