Applied Biosystems 7300 User Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration
Overview
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 37
Notes
4
Purpose of the
ROI Calibration
A regions of interest (ROI) calibration maps the positions of the wells on the sample
block so that the software can associate increases in fluorescence during a run with
specific wells of the plate. Because the instrument uses a set of optic filters to distinguish
the fluorescence emissions gathered during runs, you must generate a calibration image
for each individual filter to account for minor differences in the optical path.
ROI calibration image

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English