Ch. 4. Tutorial: AC Mode in Air, Std. Scanner Sec. 4.2. Loading Lever and Sample
20.
Check correction collar:
• Check that the green correction collar
on the objective is set to zero (this
cantilever holder has no glass window
through which the light must focus).
21.
Close enclosure door:
• Gently close the door and latch it.
22.
Motor cantilever toward sample:
• Place your eyes level with the cantilever
and sample, so you can clearly see the
gap between cantilever and sample.
• Slowly turn the ‘Engage Control Knob’
on the AFM enclosure
counterclockwise. This will lower the
cantilever holder and objective toward
the sample. The more you turn, the
faster it goes.
• Close the gap between tip and sample to
about 1 millimeter. There is no harm in
playing it safe and stopping a little
farther away. It will only cause the
automated engage to take a little longer.
Warning: Nothing but your attentiveness will
prevent the cantilever holder from crashing
into the sample. If you crash the cantilever
holder you may cause SERIOUS damage to
your cantilever holder and scanner.
23. This concludes the manual interaction with Cypher. We next turn our attention to the computer.
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