Asylum Research cypher - 6 Fluid Imaging in a Droplet

Ch. 6. Fluid Imaging in a Droplet
6. Fluid Imaging in a Droplet
CHAPTER REV. 1710, DATED 10/23/2013, 21:54. USER GUIDE REV. 1714, DATED 10/25/2013, 20:36.
Chapter Contents
6.1 Nomenclature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.2 Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.3 Preparing for Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.3.1 Mounting the Sample Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.3.2 Mounting the Cantilever . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3.3 Using the Evaporation Shield . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3.4 Sample Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.3.5 Installing the Cantilever Holder . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.3.6 Engaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.3.6.1 Pre-engage adjustments . . . . . . . . . . . . . . . . . . . . . . . 61
6.3.6.2 Focus on the cantilever . . . . . . . . . . . . . . . . . . . . . . . . 62
6.3.6.3 Focus on the sample . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.4 Imaging with the Droplet Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.4.1 AC Mode Tuning Specifics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.4.2 Imaging Specifics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.4.2.1 Engaging in fluid in AC mode . . . . . . . . . . . . . . . . . . . . . 67
6.5 Removal and Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.5.1 Removing the Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.5.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.6 Cleaning and Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.6.1 Disassembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.6.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.6.3 Reassembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.6.4 Adjusting Piezo Preload . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
6.6.4.1 Finishing up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
This chapter explains the use of the droplet cantilever holder designed for use with the Cypher Scanner.
In this design, the sample is such that the scanning area is submerged in small volume of water (typically
around 100uL) which encapsulates both the scanning area and the cantilever. The water environment is
maintained by the meniscus bridge formed between the sample substrate and the underside of the glass
window of the droplet holder.
Liquids other than water are not recommended. Volatile solvents may fill the Cypher enclosure with
damaging or harmful vapors. The membrane is made of silicone and was not designed for a high level
of chemical resistance.
BETA
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