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BIO RAD ChemiDoc Touch - Page 55

BIO RAD ChemiDoc Touch
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About Exposure Times for Chemiluminescent Images
In general, a 2x2 binning level produces images that are relatively higher in resolution,
lower in sensitivity, and require longer exposure times. An 8x8 binning level produces
images that are relatively lower in resolution and, generally, higher in sensitivity (faint
bands are optimized), and are acquired in shorter exposure times.
Tip: For a first acquisition of a sample, you might use a higher binning level (for
example, 8x8) with a shorter exposure time. Evaluate the results and then use a 2x2
binning level with a longer exposure time on the next acquisition. With some
acquisitions the 2x2 binning level might produce an image with optimal resolution and
acceptable sensitivity.
Fig. 2. Relationship between resolution and exposure time.
User Guide | 53

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