Signal Accumulation Mode
6. Enter the exposure time in seconds.
Note: You can use the exposure time from a Rapid Auto-exposure image to
estimate this exposure time.
7. Tap Done.
For more information, see The Image Resolution/Sensitivity Scale on page 49.
Signal Accumulation Mode
Signal Accumulation Mode (SAM) makes it easier to acquire high-quality
chemiluminescent images. Rather than taking a series of single images with different
exposure settings, SAM produces a series of cumulative images. Each successive image
includes all of the accumulated signal of the previous images, plus additional exposure
time.
To calculate the SAM exposure setting, estimate the shortest and the longest exposure
times you think will achieve the optimal image. Decide on the total number of images to be
taken within this time range. For example, assume you want to take four images. You
specify a minimum exposure time of 60 seconds and a maximum of 240 seconds. The first
image is taken at 60 seconds, the last image is taken at 240 seconds, and the remaining
two images are taken at even intervals in between, at 120 and 180 seconds.
SAM is useful for determining the optimal imaging time for a chemiluminescent sample.
However, the data SAM produces are not as accurate as data from a single image. Signal
near the intensity of background noise becomes increasingly masked with each
successive image. After you have used SAM to determine the optimal exposure time,
acquire a single image of the sample using the optimal exposure to identify very faint
signals in the image.
User Guide | 59
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