Chapter 4 Viewing Images
About Merging Images
A merged image consists of two images of the same sample combined into a single image.
A common example is the combination of a colorimetric image of prestained standards with
a chemiluminescent image of the same blot.
You can use the merged image to estimate the molecular weight or size of proteins on the
chemiluminescent blot. However, do not use the merged image for other types of data
analysis.
To estimate molecular weight accurately using a merged image, the images you merge
must be the same size and you must not move the sample between individual image
acquisitions.
Important: If quantitation must be accurate, export the original separate images to a
computer running Image Lab™ software and analyze them using that program.
Tip: You can select up to four images in the Gallery, switch to Image View, closely
compare them, and then choose two for merging.
Note: You can merge two images with the same binning setting or merge one image
with a 1x1 binning setting and another image with any binning setting. However, you
cannot merge two images with any other combinations of binning settings. For more
information about binning, see The Image Resolution/Sensitivity Scale on page 49 .
For more information, see Merging Images on page 85.
80 | ChemiDoc Touch Imaging System
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