Chapter 5 Excising Bands
Excising Bands from Samples
You can excise bands of interest from agarose or acrylamide gels for applications such as
mass spectrometry or DNA cloning.
The procedure for excising bands varies depending on the sample tray you use. To excise
bands on a white or blue tray, see Excising Bands on a White or Blue Tray on page 97.
Excising Bands on a Chemi/UV/Stain-Free Tray
WARNING! Transilluminators are powerful sources of UV radiation, which
can cause serious damage to unprotected eyes and skin. The accessory
UV shield (catalog #1708375) provides some UV protection. However,
this shield does not protect others standing in the area around the imager.
Before performing band excision, the user and other lab personnel in
proximity to the imager must put on protective gear including UV
protective safety glasses, a face shield, lab coat, and gloves to ensure
that no skin is exposed. A typical and reasonable expectation of use is
three operations per user a day for three minutes each. Bystanders
without protective gear must stand at least 1.5 meters (five feet) away from
the imager and limit their exposure to no longer than one hour per day.
Important: Before excising bands, you must install the UV shield.
User Guide | 91
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