Waters TQ Detector User Manual

1-12 Waters TQ Detector
Typical applications
Product ion mode is typically used for the following applications:
• Method development for MRM screening studies:
– Identifying product ions for use in MRM transitions.
– Optimizing CID tuning conditions to maximize the yield of a specific
product ion to be used in MRM analysis.
• Structural elucidation (for example, peptide sequencing)
Precursor (parent) ion mode
Precursor ion mode
Typical application
The precursor ion scan mode is typically used for structural elucidation, that
is, to complement or confirm product scan data, by scanning for all the
precursors of a common product ion.
Multiple reaction monitoring mode
MRM mode is a highly selective MS/MS equivalent of Selected Ion Recording
(SIR). Because both MS1 and MS2 are static, greater dwell time on the ions of
interest is possible and therefore better sensitivity is achievable compared
with scanning-mode MS/MS. MRM is the most commonly used acquisition
mode for quantitative analysis, allowing the compound of interest to be
isolated from the chemical background noise.
MS1
Scanning
MS2
Static (at product mass)
Collision cell
Pass all masses

Waters TQ Detector Specifications

General

Brand	Waters
Model	TQ Detector
Category	Measuring Instruments
Language	English