D-2 Preparing samples for LC/MS system check with Empower software
Assembling required materials
You must assemble the following materials before starting to prepare your
sample:
• Solution kit containing 1 mg/mL sulfadimethoxine stock in methanol.
This solution kit is provided with your instrument.
• 1 L of solvent made up of 90:10 water/acetonitrile plus 0.1% formic acid
(v/v).
• Clean class A volumetric flasks, 100-mL (3).
• Clean class A pipettes (TD), 1-mL (3).
• PTFE-sealed screw-top amber sample bottles (glass or Nalgene
®
):
125-mL (3), 15-mL.
• Sample vial, 2-mL.
Preparing the sulfadimethoxine standard
The target mass of sulfadimethoxine on the ACQUITY UPLC column is 5 pg.
Because the supplied LC/MS System Check methods specify an injection
volume of 5 µL, you must prepare a 1 pg/µL sulfadimethoxine solution.
To dilute the stock solutions to the final standard concentration:
1. Pour 1 mL of the stock solution into one of the 100-mL volumetric flasks.
2. Dilute to the 100 mL mark with solvent.
Result: The flask contains a 10 ng/µL sulfadimethoxine solution.
3. Into a second 100-mL volumetric flask, pour 1 mL of the solution created
in step 2.
4. Dilute to the 100 mL mark with solvent.
Result: The flask contains a 100 pg/µL sulfadimethoxine solution.
5. Into the final 100-mL volumetric flask, pour 1 mL of the solution created
in step 4.
6. Dilute to the 100 mL mark with solvent.
Result: The flask contains the 1 pg/µL sulfadimethoxine standard
solution.
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