Applied Biosystems 7300 User Manual

Appendix A
Decontaminating the Sample Block
112 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
14. Clean the contaminated wells of the sample
block using a small volume of 95% ethanol
solution:
a. Pipette a small volume of 95% ethanol
solution into each contaminated well.
b. In each contaminated well, pipette the
solution up and down several times to rinse
the well.
c. Pipette the ethanol solution to a waste
beaker.
15. Repeat steps 8 through 11 to rinse the wells of
the sample block and to verify that you have
eliminated the contamination.
If the contamination is still present, repeat
steps 1 through 7, then go to step 16.
IMPORTANT! Always use deionized water to
rinse wells after cleaning with bleach or ethanol
solution.
CHEMICAL HAZARD.
Sodium hypochlorite (bleach) is a liquid
disinfectant that can be corrosive to the skin and
can cause skin depigmentation. Read the MSDS,
and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and
powder-free gloves.
16. Clean the contaminated wells of the sample
block using a small volume of 10% bleach
solution:
a. Pipette a small volume of 10% bleach
solution into each contaminated well.
b. In each contaminated well, pipette the
solution up and down several times to rinse
the well.
c. Pipette the bleach solution to a waste
beaker.
95% ethanol solution

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English