9. Disinfection
NOTE 1
Containers, connectors, and hoses used for collection of samples
for microbiological analysis should be routinely cleaned and
disinfected. Soak in 200 ppm solution of bleach (0.5 ml of 5.25%
sodium hypochlorite solution per 100 ml RO water) for 10 minutes.
Rinse with RO water. Allow to air dry and store in a clean, dry
area.
NOTE 2
Because the dialysate and water connectors can be touched with
the hand and/or are exposed to air when opened, it is important
that a mid-stream sample be taken. Flush for 30 seconds prior to
taking the sample (Bland & Favero, 1990). This will ensure a
sample that reflects the true condition of the fluid pathway.
AAMI recommends sampling hemodialysis Instruments monthly
or when clinical indications suggest pyrogenic reactions and or
septicemia (AAMI, 1990, pp. 55-58). Dialysis fluid samples
should be taken at a point after the fluid leaves the dialyzer and
after the Instrument was used for a treatment (AAMI, 1990, pp.
81-94).
9.10.1.2 Dialysate Sample Collection
1. Enter Rinse Mode.
2. Press the manual bypass switch to stop flow in the dialysate
lines.
3. Disconnect the “to dialyzer” dialysate line (blue cap) from
the Instrument.
4. Without touching the dialyzer connector opening or
surrounding area, hold the connector over the fluid catchall
container, a sink, or a basin in such a manner that the
stream will flow straight out of the bottom of the connector.
(See Figure 9-1.)
Figure 9-1. Sample Collection
157-1278-916, Rev A 9-39
March 2004
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