Arena Service Manual
5. Press the manual bypass switch to start flow through the
dialyzer line.
6. Let the system flush in this manner for 30 seconds.
7. Move the sterile sample container into the stream and
aseptically collect a liquid sample.
8. Repeat Step 7 to collect a second sample.
9. Press the manual bypass switch to stop flow in the dialysate
lines.
10. Connect the dialysate line to the Instrument.
11. Press the manual bypass switch to start flow in the
dialysate lines.
12. Aseptically seal the sample containers with the lids.
9.10.2 Laboratory Procedures
All samples should be assayed within 30 minutes of collection or
refrigerated and assayed within 24 hours. Microbiological assay of
the sample should be by the pour plate technique, spread plate
technique, or membrane filtration. If the spread plate technique is
used, the sample should be quantitatively measured with a pipette
and not a calibrated loop (Bland & Favero, 1990). The calibrated
loop measurement currently being used by many laboratories does
not meet AAMI standards for determining culture counts in water
or dialysate.
Standard plate counts are the objective of the survey (AAMI, 1990,
pp. 81-94). Colonies should be counted after incubation for 48
hours at a temperature of 37° C (AAMI, 1990, pp. 81-94). If the
testing is for non-tuberculous myobacteria, the culture should be
incubated for 5 to 7 days (AAMI, 1990, pp. 81-94).
9-40 157-1278-916, Rev A
March 2004
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