BD BACTEC FX - 4.5 Entering Vials

4 – Operation
61
Preparation
At least one aerobic culture vial and one anaerobic vial should be prepared. To prepare a vial,
remove the plastic flip cap and clean the exposed rubber septum with 70% isopropyl alcohol. Use
a separate swab for each vial. Inoculate the vial with the appropriate volume of sample (refer to the
Media Package Insert for specific information on vial inoculation).
4.5 Entering Vials
To enter vials in the instrument, select a drawer where there are available stations. (The number of
available stations is shown below the vial entry icon on the Status display.)
Then follow one of the methods described below.
Vial Entry can be initiated in one of two ways:
Method 1 (Vial Activated)
• Select a drawer that has available stations, and open that drawer
• The barcode scanner turns on
• Scan a vial sequence barcode label
• The Vial Entry display appears and the Sequence, Media, and default Protocol are
automatically entered
• If you did not scan the Accession, scan or enter it now (sequence and accession can be
scanned in any order)
• To change the protocol, select Modify, then select the Up Arrow to increase or down
arrow to decrease the protocol length
• Place the vial into an available station (solid green indicator)
Method 2 (Icon Activated)
• Select a drawer that has available stations, and open that drawer
•Select vial entry on the Status display
• The Vial Entry display appears and the barcode scanner turns on
• Scan the vial sequence barcode label
• The Sequence, Media, and default Protocol are automatically entered
• If you did not scan the Accession, scan or enter it now
• To change the protocol, select Modify, then select the Up Arrow to increase or down
arrow to decrease the protocol length
• Place the vial into an available station (solid green indicator)
Note that for both methods, the Vial Entry process is not completed until the scanned vial is placed
into an available station. That is when the vial database is updated with the new vial data. The Vial
Entry tone signifies that the Vial Entry process for that vial is complete.
The barcode scanner will not turn on if the instrument does not detect the previously scanned vial
being fully inserted into a station.
When a vial is placed into the last available station in a drawer, the Activity Complete tone sounds
(3 beeps). To continue entering vials, select another drawer with available stations.
Vial Entry cannot be performed in an instrument in Isolation mode (Win FX), or an instrument in
degraded mode in a BD EpiCenter configuration.