CB (E6.1) 07/2017 Page 4/145
15. OPTIONS .............................................................................................................. 91
15.1 Communication software APT-COM™ 3 DataControlSystem (option)............................................. 91
15.2 RS 422 interface (option) .................................................................................................................. 91
15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ............ 91
15.4 Interior socket 230V (option) ............................................................................................................. 92
15.5 Analog outputs for temperature and CO
2
(option) ............................................................................ 94
15.6 Access port for extra-low voltage (option) ......................................................................................... 94
15.7 BINDER Gas Supply Service – External bottle changer for CO
2
, N
2
or O
2
(option) ......................... 95
15.8 Stands ............................................................................................................................................... 96
15.8.1 Stacking stand (option) ........................................................................................................... 96
15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ........................................... 96
15.8.3 Base on castors (option) ......................................................................................................... 96
16. REFERENCE MEASUREMENTS ........................................................................ 97
16.1 CO
2
reference measuring ................................................................................................................. 97
16.1.1 Measuring CO
2
concentration indirectly via the pH of the cell medium .................................. 97
16.1.2 Measuring CO
2
directly via chemical indicator tubes ............................................................. 98
16.1.3 Measuring CO
2
directly with an electronic infrared measuring device ................................... 99
16.2 Temperature reference measurement .............................................................................................. 99
17. AVOIDING MICROBIAL CONTAMINATION........................................................ 99
17.1 Cells and media ................................................................................................................................ 99
17.2 Laboratory conditions / equipment around the incubator .................................................................. 99
17.3 Working and behavior in the lab ..................................................................................................... 100
17.4 Chamber design and equipment of the CO
2
incubator ................................................................... 100
17.5 Handling the CO
2
incubator ............................................................................................................ 101
18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 103
18.1 Cleaning .......................................................................................................................................... 103
18.2 Decontamination / chemical disinfection of the chamber ................................................................ 105
18.3 Hot-air sterilization at 180 °C / 356 °F............................................................................................. 106
18.3.1 Overview ............................................................................................................................... 106
18.3.2 Performing a hot-air sterilization ........................................................................................... 107
18.3.3 Aborting the hot-air sterilization – general procedure ........................................................... 109
18.3.4 Aborting hot-air sterilization after less than 4 hours ............................................................. 110
18.3.5 Terminating successful sterilization during the cooling-down phase (abortion after more than
4 hours) ................................................................................................................................. 112
18.3.6 Terminating the completed sterilization cycle ....................................................................... 113
19. MAINTENANCE AND SERVICE ........................................................................ 115
19.1 Maintenance intervals, service ........................................................................................................ 115
19.2 Checking the air jacket heating fan ................................................................................................. 116
19.3 Checking the humidity system fan .................................................................................................. 116
19.4 Gas inlet fine filter ........................................................................................................................... 116
19.5 Sending the chamber back to BINDER GmbH ............................................................................... 116
20. DISPOSAL ......................................................................................................... 117
20.1 Disposal of the transport packing .................................................................................................... 117
20.1.1 Outer chamber packing ........................................................................................................ 117
20.1.2 Packing inside the chamber and equipment ......................................................................... 117
20.2 Decommissioning ............................................................................................................................ 118
20.3 Disposal of the chamber in the Federal Republic of Germany ....................................................... 118
20.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany.......................................................................................................................................... 119
20.5 Disposal of the chamber in non-member states of the EU ............................................................. 120