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Bruker AVANCE NEO User Guide
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Proton Spectrum
64 / 86
H171804E_14_001
8.6
Optimizing the Sweep Width
Optimizing the
sweep width will
achieve a better
resolution and
separation of the
peaks in
the
Proton spectrum.
•
On the toolbar, click
Exact Zoom
.
•
Enter the following F1 [ppm] values:
From = 4.6
To = 3.6
•
In the exactzoom window, click
OK
.
63
65
Table of Contents
Table of Contents
3
1 Introduction
5
Sources of Hazards
5
Software Version and Command Syntax
5
2 Safety
7
Magnetic Safety
7
Cryogenic Safety
7
Electrical Safety
7
Chemical Safety
8
CE Certification
8
3 Introductory Theory and Terminology
9
Figure 3.1: Excitation and Response
9
Figure 3.2: NMR Spectrum
10
NMR Analysis of Chloroform
11
Figure 3.3: NMR Analysis of CHCI3
11
Figure 3.4: NMR Signals Emitted by CHCI3
12
Figure 3.5: Converting Hertz and Ppm
13
Proton NMR - Chemical Shift
14
Figure 3.6: 1H Chemical Shifts in Organic Compounds
14
Proton Spectrum of Benzene
15
Figure 3.7: Benzene Ring
15
Figure 3.8: Spectrum of Benzene
15
Proton Spectrum of Benzylacetate
16
Figure 3.9: Benzylacetate
16
Proton Spectrum of Ethylbenzene with Spin/Spin Coupling
17
Figure 3.10: Proton Spectrum of Benzylacetate
17
Figure 3.11: Ethylbenzene
18
Figure 3.12: Ethylbenzene Spectrum
18
Decoupling
19
Figure 3.13: Decoupling Experiment
20
Figure 3.14: Ethylbenzene Spectrum with Homodecoupling
20
FID and Spectrum
21
Figure 3.15: Fourier Transformation
21
4 System Description
23
Figure 4.1: AVANCE NEO Console and Ascend Magnet
23
AVANCE Architecture Overview
24
Operator Console and Connections
24
Figure 4.2: AVANCE NEO Architecture Overview
24
Console
25
Link between the Host Computer and the AQS
26
Magnet, Shim System, HPPR and Probe
26
Figure 4.3: Photo of Magnet, Shim System, Probe and HPPR
26
The Magnet and Magnet Dewar
27
Room Temperature Bore
28
Figure 4.4: Superconducting Magnet
28
Helium Tank
29
Nitrogen Tank
29
Introduction to the Lock System
29
Probes
30
Figure 4.5: Sample in Probe
30
Figure 4.6: Typical HPPR Cabling
31
Broadband Probe
32
Figure 4.7: Example of a Broadband Probe
32
Iprobe
33
Figure 4.8: the Bruker Iprobe Platform
33
Changing a Probe
34
5 Basic Procedures
35
The Topspin Window
35
Creating a New Data Set
36
Sample Preparation
38
Figure 5.1: Inserting the Sample into the Spinner
38
Inserting the Sample Plus Spinner into the Magnet
39
Locking the Sample
39
Figure 5.2: Solvents Table
39
Tuning and Matching the Probe
40
Probes Equipped with ATM Using the Automated Tuning Routine
40
Figure 5.3: Lock Display after Locking the Sample
40
Probes Equipped with ATM Using the Manual Tuning Routine
41
Figure 5.4: ATMM Probe Tuning/Matching Window
41
Spinning the Sample
43
Figure 5.5: Examples of Wobble Curves with Different Tuning and Matching
43
Shimming
44
Routine Shimming Using Topshim
44
Setting the Probe/Solvent Dependent Parameters
44
Adjusting the Receiver Gain
44
Starting the Acquisition
44
Processing the Data
45
Figure 5.6: the Proc1D Window
45
6 Preparing for Acquisition, Frequency Related Parameters
47
Frequency
47
Numerical Explanation of Transmitted, Basic and Offset Frequencies
48
Figure 6.1: Spectrum with BF1 = 600.13 Mhz, 01 = 0 Hz
48
Figure 6.2: Spectrum with BF1 =600.13 Mhz, 01= 8 Khz
49
Figure 6.3: Spectrum with BF1 = 600.13 Mhz, 01 = 8 Khz, SWH = 8.4 Khz
49
Figure 6.4: Interaction of SFO1, BF1 and O1
50
7 The NMR Sample
51
Solvent Selection
51
Sample Tube
52
Figure 7.1: Spectrum Showing Spinning Sidebands
52
Sample Handling
53
8 Proton Spectrum
55
Experiment Setup
55
Acquisition
59
Processing
59
Integration
61
Plotting the 1D Proton Spectra
63
Optimizing the Sweep Width
64
9 ¹³C Spectrum with Proton Decoupling
67
Experiment Setup
68
Acquisition
70
Processing
70
Peak Picking
72
Plotting the 1D Carbon Spectrum
73
10 Contact
75
List of Figures
77
List of Tables
79
Glossary
81
Index
83
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Bruker AVANCE NEO Specifications
General
Brand
Bruker
Model
AVANCE NEO
Category
Laboratory Equipment
Language
English
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