Bruker AVANCE NEO - Table of Contents

Contents
H171804E_14_001
iii
Contents
1 Introduction.........................................................................................................................................5
1.1 Sources of Hazards ............................................................................................................5
1.2 Software Version and Command Syntax ............................................................................5
2 Safety...................................................................................................................................................7
2.1 Magnetic Safety ..................................................................................................................7
2.1.1 Cryogenic Safety.................................................................................................................7
2.2 Electrical Safety ..................................................................................................................7
2.3 Chemical Safety..................................................................................................................8
2.4 CE Certification ...................................................................................................................8
3 Introductory Theory and Terminology .............................................................................................9
3.1 NMR Analysis of Chloroform.............................................................................................11
3.2 Reference Compounds, Hertz, ppm .................................................................................13
3.3 Proton NMR - Chemical Shift............................................................................................14
3.4 Proton Spectrum of Benzene............................................................................................15
3.5 Proton Spectrum of Benzylacetate ...................................................................................16
3.6 Proton Spectrum of Ethylbenzene with Spin/Spin Coupling .............................................17
3.7 Decoupling ........................................................................................................................19
3.8 FID and Spectrum.............................................................................................................21
4 System Description ..........................................................................................................................23
4.1 AVANCE Architecture Overview .......................................................................................24
4.1.1 Operator Console and Connections..................................................................................24
4.2 Console.............................................................................................................................25
4.3 Link between the Host Computer and the AQS ................................................................26
4.4 Magnet, Shim System, HPPR and Probe .........................................................................26
4.5 The Magnet and Magnet Dewar .......................................................................................27
4.5.1 Room Temperature Bore ..................................................................................................28
4.5.2 Helium Tank......................................................................................................................29
4.5.3 Nitrogen Tank ...................................................................................................................29
4.6 Introduction to the Lock System........................................................................................29
4.7 Probes...............................................................................................................................30
4.8 Broadband Probe..............................................................................................................32
4.9 iProbe................................................................................................................................33
4.10 Changing a Probe .............................................................................................................34
5 Basic Procedures .............................................................................................................................35
5.1 The TopSpin Window........................................................................................................35
5.1.1 Creating a New Data Set ..................................................................................................36
5.1.2 Sample Preparation ..........................................................................................................38
5.2 Inserting the Sample plus Spinner into the Magnet ..........................................................39
5.3 Locking the Sample ..........................................................................................................39
5.4 Tuning and Matching the Probe........................................................................................40
5.4.1 Probes Equipped with ATM using the Automated Tuning Routine ...................................40