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9.5 Tissue processing selection guidelines
Ethanol-Isopropanol-WAX
1. Ethanol-Isopropanol recommended as the standard routine method for small to medium-large size
biopsies.
2. Fat content of the tissue is kept to a minimum (< 2mm thickness).
3. Very Cost effective for general tissue-processing.
4. Strongly recommended for processing of Renal/Gastric, breast core biopsies and equivalent tissue
size.
Eth-JFC-Isopropanol-WAX/ JFC-Isopropanol
1. JFC-ISO sequence is strongly recommended for high fat content such as Breast / Lipoma up to full
size cassette in less than 4 hours.
PROWAVE-WAX
2. PROWAVE is a “one step” solution that can be used for tissues where overnight fixation time is
uncertain, since PROWAVE can also act as a secondary fixative.
3. Ideally suited for non-fatty tissues (up to 5mm thick) or moderate lipid content tissues (up to 3mm
thick).
Ethanol-Xylene-Isopropanol-WAX
1. Ethanol-Xylene is not recommended from Milestone, is up to the user to decide the use of xylene. It
can be used as the standard routine method for small to medium-large size biopsies.
Ethanol-Isoparaffin (MileGREEN®)-Isopropanol-WAX
1. Isoparaffin (MileGREEN®)-ISO sequence is recommended for high fat content such as Breast /
Lipoma.
Refer to chapter 3.3.1 Tanks for process and main cavity cleaning, for suggestions on how
to set up tanks in LOGOS ONE.
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