Milestone LOGOS One - Page 117

Milestone s.r.l.
MM098-002 – LOGOS ONE – Operator Manual
117
TISSUE PROCESSING SELECTION GUIDELINES
Washing in
Ethanol 1 min
FRESH RENAL BIOPSIES
Soaking in Formalin up to 30 min.
Heating in Formalin
5 min. ramping time to 50°C
10 min. holding at 50°C
Ethanol 100% - 10 min total time.
Isopropanol 100% - 10 min total time.
WAX 15-20 min.
PARTIALLY FIXED
TISSUE BLOCKS
FIXED TISSUE
10/20’: Small biopsy
30’: 3mm
60’: 5mm
Heated Fixation
complete phase
FRESH TISSUE
30’: Small biopsy
1 hr: 3mm
1.5 hr: 5mm
2/3 hrs:5mm breast/fatty tissues
Soaking in Formalin
with agitation
Rinsing in Ethanol
Ethanol
JFC
Isopropanol
Routine
method
Fatty
tissues
ProWave
(o JFC)
One step
routine
Isopropanol
WAX