MM098-002 – LOGOS ONE – Operator Manual
Collapse of block
upper face after
sectioning.
Tissue block surface appears
to collapse “Dry back” when
the tissue is observed the
next day.
This occurs even though a
section of the block have
been easily cutted the
previous day.
Tissue block not difficult to
section the same day.
1. Tissue has been sufficiently
dehydrated and cleared (dry tissue)
allowing sections to be cut, however
WAX impregnation is not entirely
complete with residual amounts of the
previous solution remaining.
Indicated by continued bubbling originating
from the tissue blocks in the final stage (near
the end) of the WAX program.
Does not occur in smaller blocks, but may
appear as tissues increase in size. The
result is that tissues that retain any of the
solvent will escape by next day, producing
this effect, varying according to the size.
1. Retrospectively increase WAX impregnation
time by melting the block down and placing it
into molten WAX in a WAX oven for
convenience. At least an hour.
AND
Prospectively increase standard WAX
impregnation times. Approx. 30 minutes for
the longest processing program. For the
shorter processing programs approximately
15 minutes increase in WAX time.
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