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NOT GOOD WETNESS
A. Follow the recovery laboratory internal protocols for air dried samples.
B. If not available, follow Milestone suggested recovery protocol.
C. Check if the unit can be used for a new cycle (*).
D. Restart the processing cycle.
1. Absolute alcohol 2 changes, 1 hour each.
2. 95% alcohol 2 changes, 1 hour each.
3. Running tap water for 30 minutes.
4. Place in formol-glycerol solution (*) until tissues become soft and pliable with gentle pressure.
Tissues may remain in formol-glycerol for up to 8 hours without adverse results.
5. Reprocess on tissue processor in usual manner.
If samples have been put in WAX, it is necessary to start with the dewaxing step:
Xylene, 3 changes, 1 hour each
Formol-Glycerol Working Solution:
Formalin- Sodium Acetate, (Stock) 90ml
Glycerin (Glycerol) 10ml
Formal-Sodium Acetate (Stock):
Formaldehyde, 38-40% 10ml
Sodium Acetate 2.0 gm
Tap Water 90ml
(*) Should Formol-Glycerol not be available, use tap water, even if the quality of the recovery is lower.
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