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A6 LIGHTSHEET SCANNING MODE
The pco.edge Lightsheet scanning mode is a
special mode for lightsheet microscopy and only
available for Camera Link versions via SDK (not
in Camware).
It is based on Rolling Shutter mode and uses the
readout mode single top down (Mode E see
6.9.3).
Standard line time (see 1.2) value is 40 µs 2 and it
can be set from camera specific line time (see 1.2)
up to 100 ms. An exposure area (between the
orange bars, e.g. five lines 3 ) can be user
defined. It is possible for the user to select the
number of lines and the exposure time per line.
This user-defined capability makes it possible to
synchronize the pco.edge cameras with a
lightsheet microscope that requires this method of
camera exposure timing
Workflow (only PCO SDK)
• Set Rolling Shutter mode
• Set readout format E
• Set line timing on (0x0001 ≙ a CMOS_LINETIMING_PARAM_ON
see sc2.defs.h) and set the appropriate line time
• Set number of exposure lines
• Set number of delay lines
• Trigger mode and acquire mode and hwio settings are free to
select by the user
SDK settings: (see SDK manual)
SC2_SDK_FUNC int WINAPI
PCO_SetCmosLineTiming
wTimeBase (e.g. µs )
SC2_SDK_FUNC int WINAPI
PCO_SetCmosLineExposureDelay
dwExposureLines (e.g. 5 )
dwDelayLines (e.g. 4 )