Roche Cedex Bio User Manual

Roche Diagnostics
C-12
Operator’s Manual · Version 3.4
8 Data analysis methods Cedex Bio analyzer
Photometric measurements
To avoid values that are too low when calculating the kinetics, you can perform the
following checks with floating limits (test parameters):
With ascending reaction curve:
and
With descending reaction curve:
and
If the result for is negative, set the value for this
expression to zero. In such a case, the floating reaction limit becomes a fixed reaction
limit.
Calculation of the absorbance
difference per minute
This calculation is performed with the Kinetics calculation model.
With the specified points (at least 2) a Passing/Bablok analysis is carried out to
determine the absorbance difference per minute (Kinetic).
Nonlinear check This check is used with the Kinetics calculation model.
The linearity of the curve is determined by analyzing the points which lie within the
limits specified for the linear region. A Passing/Bablok analysis is carried out on each
half of the curve to determine the straight lines. The slopes of these straight lines are
determined using the differences ΔA1 and ΔA2.
If there is an even number of points within the linear range the two halves of the
curve overlap at two points.
Conditions for running the test:
Test conditions:
The Non Linear flag is displayed if the conditions are not fulfilled.
Corrected Reaction Limit Low (Floating RL Low, test parameter).
Corrected Reaction Limit High (Floating RL High, test parameter).
Sample absorbance value of a cuvette (test parameter).
Calibrator absorbance value of a reference calibrator cuvette (test
parameter).
fl. RL
low
RL
low
A
cuv
sample()A
cuv
cal
ref
()–()+=
fl. RL
high
RL
high
=
fl. RL
low
An() fl. RL
high
≤≤
fl. RL
high
RL
high
A
cuv
sample()A
cuv
cal
ref
()–()+=
fl. RL
low
RL
low
=
fl. RL
low
An() fl. RL
high
≤≤
A
cuv
sample()A
cuv
cal()–()
fl. RL
low
fl. RL
high
A
cuv
sample()
A
cuv
cal
RF
()
Absorbance change (ascending) of the first half of the curve.
Absorbance change (ascending) of the second half of the curve.
Linearity in Δ (ΔA/min) (linearity test parameter).
Rate 0.006abs/min>
ΔA
1
min⁄ΔA
2
min⁄– lin<
ΔA
1
ΔA
2
lin

Roche Cedex Bio Specifications

General

Brand	Roche
Model	Cedex Bio
Category	Measuring Instruments
Language	English