Roche Diagnostics
Operator’s Manual · Version 3.4 C-3
Cedex Bio analyzer 8 Data analysis methods
Table of contents
Data analysis methods
Measurement principles
In this chapter, you will find information on the measurement principles used with
Cedex Bio analyzer, and it describes the calculations and checks executed when
performing sample, calibration, and control measurements.
Photometric measurements ............................................................................................C–5
Absorbance .................................................................................................................C–6
Calculation of absorbance ..................................................................................C–6
Cuvette blank correction .....................................................................................C–6
Volume correction ...............................................................................................C–7
Absorbance checks ...............................................................................................C–8
Rate ..............................................................................................................................C–9
Calculation model Endpoint ........................................................................... C–10
Calculation model Kinetic ............................................................................... C–11
Reaction direction ............................................................................................ C–13
Antigen Excess check ....................................................................................... C–14
Test Range check (level rate) ........................................................................... C–16
Calculation of mean rate .................................................................................. C–16
Quantitative calibration ......................................................................................... C–17
Calibration mode Linear Interpolation ......................................................... C–17
Calibration mode Linear Regression .............................................................. C–18
Calibration mode Logit/Log 4 ......................................................................... C–19
Calibration mode Logit/Log 5 ......................................................................... C–20
Calibration mode Exponential 5 ..................................................................... C–21
Calibration checks ............................................................................................ C–21
Results ....................................................................................................................... C–23
Quantitative result calculation ........................................................................ C–23
Corrections ........................................................................................................ C–25
Calculation of replicate mean .......................................................................... C–26
Result calculation checks ................................................................................. C–26
Laboratory conversion ..................................................................................... C–28
Quality control ............................................................................................................... C–29
In this chapter
Chapter
8
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