Roche Cedex Bio - Page 334

Roche Diagnostics
C-8
Operator’s Manual · Version 3.4
8 Data analysis methods Cedex Bio analyzer
Photometric measurements
Absorbance checks
High Absorbance check This check is carried out for every absorbance calculation.
All absorbencies used for the calculation of all measuring points of all requested
wavelengths between the first and last point may not exceed 2.0 absorbance units.
The first and last points refer to:
o (Endpoint and Kinetic)
o (High/Low Activity)
o (Antigen Excess)
The High Abs flag is displayed if a value between the first and last points exceeds 2.0.
Reagent Range check This check is carried out for every absorbance calculation for the calibrator with the
lowest concentration.
For the reagent blank, all absorbencies required for calculations are checked to ensure
they do not exceed the preset reagent range limits.
The < Reag Rng or > Reag Rng flag is displayed if the absorbance of the reagent in a
cuvette is not within the specified range.
High Activity check This check is used to identify large differences in the absorbance after the start of the
reaction.
With ascending reaction curve:
With descending reaction curve:
The High Act flag and the value of the upper test range limit are displayed in the
result list if the value does not meet the conditions.
Absorbance of each point (n) (between the first and last points) at
requested wavelength λ.
Calc
first
…Calc
last
HighAct
first
…HighAct
last
Ag
first
…Ag
last
A
raw cuv
λ n,() 2.0≤
A
raw cuv
λ n,()
Absorbance of the reagent blank of each point used in the
calculations.
Reagent range lower limit (test parameter).
Reagent range upper limit (test parameter).
RR
low
A
cuv
n() RE
high
≤≤
A
cuv
n()
RR
low
RR
high
Absorbance at the time of the high activity first point (test
parameter).
Absorbance at the time of high activity last point (test parameter).
High activity limit (test parameter).
A
cuv
last()A
cuv
first() HLL≤–
A
cuv
first()A
cuv
last() HLL≤–
A
cuv
first()
A
cuv
last()
HLL